Database : HANSEN Search on : MYCOBACTERIUM LEPRAE/GENET [Subject descriptor] References found : 130 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 19844 Author: Cole, Stewart T. Title: The Mycobacterium leprae genome projest. Source: Int. J. Lepr;66(4):589-591, Dec. 1998. . Conference: Present in: International Leprosy Congress, 15, Beijing, 07-12 Sept. 1998. Descriptors: Mycobacterium leprae/genet DNA Bacteriano/quim Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1998/pdf/v66n4/v66n4repcur03.pdf / en Location: BR191.1

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Id: 19802 Author: Yogi, Yasuko; Endoh, Masumi; Banba, Tomoko; Okamura, Haruki; Nomaguchi, Hiroko. Title: Susceptibility to Mycobacterium leprae of ALY (Alymphoplasia) mice and IFN-gamma induction in the culture supernatant of spleen cells. Source: Int. J. Lepr;66(4):464-474, Dec. 1998. ilus, graf. Abstract: The aly/aly (alymphoplasia) mice from a mutation of a colony of the C57BL/6J mouse strain, which has a systemic absence of lymph nodes and Peyer's patches, are deficient in both T- and B-cell-mediated immune functions. We have undertaken a comparison of susceptibility to Mycobacterium leprae of ALY (aly/aly, aly/+) mice with C57BL/6J mice. The aly/aly mouse was found to have an excellent high susceptibility to M. leprae with no distinction between female and male. The aly/+ mouse also was more susceptible to M. leprae at an earlier stage than the C57BL/6J mouse. Therefore, we examined and compared the cytokine gene expression and gamma interferon (IFN-gamma) induction in the splenocytes of ALY mice. The expression of interleukin 4 (IL-4), IL-10 and IL-12 mRNA was weakly stimulated with ML-lysate in inoculated aly/aly mice but IL-2, IL-6, IGIF/IL-18 and IFN-gamma mRNA were not observed. None of the cytokine genes used appeared, except the mRNA for IL-1-alpha, when uninfected cultured spleen cells were stimulated with ML-lysate. Also, IFN-gamma production was not induced. However, the appearance of these cytokine genes was observed when stimulated with concanavalin A (ConA), and IFN-gamma production was also induced in the culture supernatant by aly/+ and even aly/aly mice stimulated with ConA. To examine the reason why IFN-gamma cannot be produced by splenocytes of ALY mice inoculated with M. leprae, we detected cytokine gene expression and IFN-gamma induction in the presence of recombinant murine IL-12 or IGIF/IL-18. IL-2 mRNA expression was detected in all of the mice tested in the presence of IL-12 but not in aly/aly mice under IGIF/IL-18, and iNOS mRNA expression was not observed in aly/aly mice under IL-12 or IGIF/IL-18. IL-4 and IL-10 mRNA were detected by aly/aly mice only by exposure to IGIF/IL-18. In culture, the supernatant with ML antigens of the aly/aly mice did not produce IFN-gamma in spite of the presence of IL-12 and IGIF/IL-18, while IFN-gamma was weakly induced in aly/+ mice stimulated with ML-lysate and in the presence of IGIF/IL-18. Nevertheless, IFN-gamma production was observed in splenocytes of the aly/aly mice stimulated with ConA and also with IGIF/IL-18 plus anti-CD3 antibody. Our results suggest that ALY mice might be showing a high susceptibility to M. leprae because of deficient priming for activation of T cells with the leprosy bacilli infection. Moreover, it is possible that the phagocytic activities of the macrophages of ALY mice are also impaired. (AU)^ien. Descriptors: Hanseníase/genet Hanseníase/imunol Mycobacterium leprae/genet Mycobacterium leprae/imunol Limits: Animais Camundongos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1998/pdf/v66n4/v66n4a04.pdf / en Location: BR191.1

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Id: 19741 Author: Khanolkar, Saroj R; Mackenzie, Charles D; Lucas, Subastein B; Hussen, Armal; Girdhar, Bhawneshwar K; Katoch, Kiran; Mcadam, Keith PWJ. Title: Identification fo Mycobacterium leprae antigens in tissues of leprosy patients using monoclonal antibodies. Source: Int J Lep;57(3):652-658, sept. 1989. ^btab, ^bilus. Abstract: Five monoclonal antibodies (MAbs) directed against antigens of Mycobacterium leprae were tested for their ability to bind to components of tissue sections prepared from biopsies taken from patients with various forms of leprosy. Immunoperoxidase was the most successful marker system used, although immunofluorescence and alkaline phosphatase were also successful in certain cases. Positivity was high with all five antibodies successfully staining those sections containing a bacterial index of 3+ or more; sections with 0 bacterial counts also had areas staining positively with two of the MAbs. The positive staining in the tissues was confined to areas infiltrated by inflammatory cells; however it was not identifiable as being associated with individual bacteria. These findings suggest that immunostaining with specific monoclonal antibodies can help to identify leprosy in diagnostic samples in which acid-fast bacilli are not identifiable by standard histochemical means. Immunohistochemical techniques are likely to be valuable in studies of the distribution of M. leprae antigens and their association with individual tissue elements^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Anticorpos Monoclonais/genet Anticorpos Monoclonais/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n3/v57n3a09.pdf / en Location: Br191.1

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Id: 19735 Author: Katoch, Vishwa M; Katoch, Kiran; Ramanathan, Usha; Sharma, Vishnu D; Shivannavar, Chinappa T; Datta, Arun K; Bharadwaj, Ved P. Title: Effect of chemotherapy on viability of Mycobacterium leprae as determined by ATP Content, morphological index and FDA-EB fluorescent staining. Source: Int J Lep;57(3):615-620, sept. 1989. ^btab. Abstract: Viable bacterial populations were estimated in bacilli purified from 105 biopsies from 40 untreated and 65 multibacillary leprosy patients treated with multidrug therapy (MDT) for varying periods. The bacilli were purified and viability was determined by ATP content, morphological index (MI), and fluorescein diacetate-ethidium bromide (FDA-EB) staining. Viable populations were calculated, taking 3.58 x 10(-15) g/solid bacillus as the mean ATP content of a viable unit of Mycobacterium leprae. The proportion of viable bacilli was also estimated in the same specimens using solid-staining (MI) and green-staining bacilli by the FDA-EB method. In the untreated cases, the positive viability by ATP assay was 100%, 92% by MI, and 100% by FDA-EB. ATP content per solid bacillus was relatively constant, which was not the case with ATP content per green-staining bacillus. While the MI was zero in all cases, viable bacilli could still be detected by ATP estimations in 5 of the 32 (16%) patients after 2 years of MDT and in 1 of the 20 (5%) patients after 3 years of MDT. No viable bacilli could be detected even by this method beyond 3 years of MDT. On the other hand, green-staining bacilli were demonstrable in 7/32 (22%) of cases after 2 years of MDT, 2/20 (10%) after 3 years of MDT, and 1/13 (8%) after more than 3 years of treatment, indicating that the FDA-EB staining and ATP assay did not detect the same populations. A determination of the ATP content of M. leprae could be used as a reliable and sensitive tool for determining viability of the bacilli^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Trifosfato de Adenosina/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1989/pdf/v57n3/v57n3a03.pdf / en Location: Br191.1

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Id: 19708 Author: Soto, Carlos Y; Moreno, Pedro A; Valencia, James T; Bernal, Maria M; Guzman, Fanny; Patarroyo, Manuel E; Murillo, Luis A. Title: Isolation, characterization, molecular cloning and amplification of a species-specific M. leprae antigen. Source: Int. J. Lepr;67(4):392-402, Dec., 1999. ilus, tab, graf. Abstract: A polyclonal serum sample from a lepromatous leprosy (LL) patient, which presented a specific recognition pattern for leprosin, was used to screen a Mycobacterium leprae genomic library constructed with DNA isolated from human lepromas. One clone, designated ML4-1, which expressed a specific antigenic determinant of M. leprae as part of a beta-galactosidase fusion protein, was isolated. The 1.932 bp M. leprae-derived genomic fragment was sequenced, and it had an incomplete open-reading frame shown to code for a 644 amino-acid polypeptide (72.3 kDa). Some partial nucleotide homology to the M. tuberculosis MTCY9C4 cosmid and the M. leprae B1913 cosmid were found. Southern blot assays using the 584 bp Eco RI-Bam HI fragment excised from the ML4-1 clone revealed that this sequence is present only in the M. leprae genome and not in the 24 different mycobacterial DNA tested. Two oligonucleotides based on the genomic sequence were also synthesized and used as amplifiers for a polymerase chain reaction (PCR) test, giving a positive signal exclusively in M. leprae DNA. Furthermore, 32 sequential synthetic peptides, 20 amino-acids long, spanning the entire protein corresponding to the hypothetical ML4-1 clone sequence, were synthesized and evaluated by ELISA. A peptide included in the 221-240 region was significantly recognized by either lepromatous leprosy or healthy tuberculosis contact patient sera. Thus, PCR amplification of this fragment, along with the recognition of its protein sequence by leprosy patient sera, could be a useful tool for a potential diagnostic method in the detection of M. leprae infection in the future. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/isol Hanseníase Hanseníase/genet Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n4/v67n4a04.pdf / en Location: Br191.1

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Id: 19690 Author: Sticht- Groh, V; Bretzel, G; Harmsen, D; Heesemann, J; Ishaque, M. Title: Correspondence: An Unusual Spreading Phenomenon in an Atificial Medium Inoculated with M. leprae. Source: Int. J. Lepr;63(1):100-101, 1995. ^bilus. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Location: BR191.1

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Id: 19682 Author: World Health Organization. Title: Analysis of Vaccines Prepared from Armadillo- Derived M. leprae; Results of an Inter- Laboratory Study Coordinated by the World Health Organization. Source: Int. J. Lepr;63(1):48-55, 1995. ^bilus, ^btab, ^bgraf. Abstract: Resumo: Preparations of armadillo-derived Mycobacterium leprae used in vaccine trials were analyzed using a combination of morphological, chemical and immunological criteria. When compared to more recent preparations, vaccine lots prepared in 1984 and 1985 were found to contain fewer intact bacilli and lower amounts of M. leprae antigens. These differences may be characteristic of the original preparations, or alternatively, may have arisen during prolonged storage. The early vaccine lots were those used in the recently published Venezuela trial. Descriptors: Mycobacterium leprae/genet Hanseníase/quimioter Organização Mundial da Saúde Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1a08.pdf / en Location: BR191.1

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Id: 19680 Author: Misra, Namita; Ramesh, Venkatesh; Misra, Radhey S; Narayan, N. P. S; Colston, Michael Joseph; Nath, Indira. Title: Clinical Utility of LSR/A 15 Gene for Micobacterium leprae Detection in Leprosy Tissues Using the Polymerase Chain Reaction. Source: Int. J. Lepr;63(1):35-41, 1995. ^bilus, ^btab. Abstract: Resumo: Skin biopsy and slit-skin smears from 46 leprosy patients and 4 nonleprosy patients were tested for the presence of Mycobacterium leprae by the polymerase chain reaction (PCR) using primers based on the sequence of the LSR/15 kD gene. The PCR was found to be specific and sensitive, with a detection level of 10 and 100 bacilli. PCR using skin biopsies gave a higher detection rate than did slit-skin smears, probably due to the higher density of bacilli in a 4-mm punch biopsy. Dot blot hybridization with radioactive probes was 10-fold more sensitive than the ethidium bromide staining. Eight patients who did not show acid-fast bacilli in tissues by the conventional methods were shown to have PCR-amplified M. leprae DNA. False-negative results were obtained in 3 cases even though formal evidence for tissue inhibitors was absent. Descriptors: Mycobacterium leprae/genet Hanseníase/compl Hanseníase/diag Hanseníase/fisiopatol Reação em Cadeia da Polimerase/métodos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1a06.pdf / en

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Id: 19679 Author: Nakamura, Masahiro. Title: Optimal pH for Preserving the Activity of Mycobacterium leprae During Incubation of Cells in a Cell-Free Liquid Medium. Source: Int. J. Lepr;63(1):28-34, 1995. ^bgraf. Abstract: Resumo: The effect of the pH of a cell-free liquid medium on the activity of Mycobacterium leprae during incubation of the cells was investigated. As a parameter for evaluating the activity, the amount of adenosine triphosphate (ATP) extracted from the incubated cells collected by centrifugation was measured. The results demonstrate that the activity of M. leprae cells was maintained at a significant level for approximately 4 weeks at 30 degrees C in 0.05 M phosphate buffer containing 10% fetal calf serum at pH 7.0 compared to cells at other pHs tested, but activity was not preserved in phosphate buffer at pH 7.0 without serum and incubated at 37 degrees C. The maintenance of the activity under these conditions was prolonged somewhat by the addition of glycerin (2%) to the medium, and was definitely inhibited by rifampin but not by either penicillin or isoniazid. From the results reported here, it could be postulated that the optimal pH of cell-free media for the study of cultivation of M. leprae is 7.0. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Hanseníase/genet Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1995/pdf/v63n1/v63n1a05.pdf / en Location: BR191.1

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Id: 19666 Author: Ngamying, Maeya; Levy, Louis; Brennan, Patrick J. Title: Vaccination of mice against the leprosy bacillus with skin-test antigens. Source: Int. J. Lepr;67(3):305-307, Sept., 1999. . Descriptors: Antígenos/uso diag Antígenos/imunol Mycobacterium leprae/genet Mycobacterium leprae/imunol Limits: Camundongos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3cor02.pdf / en Location: BR191.1

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Id: 19661 Author: Roche, Paul W; Failbus, Sarah S; Britton, Warwick J; Cole, Robert. Title: Rapid method for diagnosis of leprosy by measurements of antibodies to the M. leprae 35-kDa protein: comparison with PGL-1 antibodies detected by ELISA and "Dipstick" methods. Source: Int. J. Lepr;67(3):279-286, Sept., 1999. ilus, graf. Abstract: A new rapid immuno-chromatographic test card for the detection of antibodies to the Mycobacterium leprae 35-kD protein is described. The new assay is compared in the same group of subjects with a direct enzyme ELISA method for 35-kD antibodies and with assays for anti-phenolic glycolipid-I (PGL-I) antibodies using a standard ELISA as well as the recently described [quot ]dipstick[quot ] method. Good concordance was found between the rapid methods and the corresponding ELISA methods. The detection of untreated paucibacillary leprosy by the 35-kD test card was 59% compared with 27% for the PGL-I dipstick; however, the specificity for the 35-kD test card was 90% compared with 100% for the PGL-I dipstick in an endemic population. The potential application of these new, rapid serologic methods for the diagnosis of leprosy under field conditions is discussed. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/fisiol Anexina A3/imunol ELISA/métodos Limits: Humanos Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a07.pdf / en Location: BR191.1

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Id: 19659 Author: Sharma, Pankaj; Kar, Hemant K; Misra, Radhey S; Mukherjee, Ashok; Kaur, Harvinder; Mukherjee, Rama; Rani, Rajni. Title: Induction of lepromin positivity following immuno-chemotherapy with mycobacterium w vaccine and multidrug therapy and its impact on bacteriological clearance in multibacillary leprosy: report on a hospital-based clinical trial with the candidate antileprosy vaccine. Source: Int. J. Lepr;67(3):259-269, Sept., 1999. tab, graf. Abstract: A vaccine based on autoclaved Mycobacterium w was administered, in addition to standard multidrug therapy (MDT), to 157 bacteriologically positive, lepromin-negative, multibacillary (LL, BL and BB) leprosy patients. The vaccinees were supported by a well-matched control group of 147 patients with similar type of disease who received a placebo injection in addition to MDT. The MDT was given for a minimum period of 2 years and continued until skin-smear negativity, while the vaccine was given at 3-month intervals up to a maximum of 8 doses. The lepromin response evaluated in terms of percentage of subjects converting to positivity status, measurement in millimeters, and duration of lepromin positivity sustained, reflected a statistically significant better outcome in the vaccine group patients (especially LL and BL leprosy) in comparison to those in the placebo group. The data indicate that lepromin-positivity status seems to have an impact on accelerating the bacteriological clearance, as is evident by the statistically significant accelerated decline in the BI of those patients who converted to lepromin positivity as compared to those remaining lepromin negative throughout therapy and post-therapy follow up. To conclude, the addition of the Mycobacterium w vaccine to standard MDT induces a lepromin response of a statistically significant higher magnitude than that observed with MDT alone. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Hanseníase/genet Hanseníase/imunol Antígeno de Mitsuda/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a05.pdf / en Location: BR191.1

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Id: 19658 Author: Sharma, Pankaj; Kar, Hemant K; Misra, Radhey S; Mukherjee, Ashok; Kaur, Harvinder; Mukherjee, Rama; Rani, Rajni. Title: Disabilities in multibacillary leprosy following multidrug therapy with and without immunotherapy with mycobacterium w antileprosy vaccine. Source: Int. J. Lepr;67(3):250-258, Sept., 1999. tab. Abstract: A vaccine based on autoclaved Mycobacterium w was administered, in addition to standard multidrug therapy (MDT), to 157 bacteriologically positive, lepromin-negative, multibacillary leprosy patients supported by a well-matched control group of 147 patients with similar type of disease who received a placebo injection in addition to MDT. The MDT was given for a minimum period of 2 years and continued until skin-smear negativity, while the vaccine/placebo was given at 3-month intervals up to a maximum of 8 doses in the initial 2 years. The overall incidence of type 1 and type 2 reactions and neuritis during treatment and follow up was nearly equal in the patients in the vaccine and placebo groups; the differences were not statistically significant. The occurrence of disabilities, such as anesthesia, trophic ulcers, claw hand and grade 3 deformities, were not different statistically in the vaccine and placebo groups, an observation valid both for deformities present at induction and for those which developed during the course of therapy and surveillance. A statistically significant difference was observed in the recovery of newly developed trophic ulcers; recovery was quicker in the vaccine group. The recovery rate for motor deformities was marginally higher in the vaccine group, although not significant (p = 0.068) statistically. There was a statistically significant reduction in the incidence of grade 3 deformities following MDT with and without immunotherapy. To conclude, the addition of vaccine to MDT did not precipitate neuritis or deformities over and above that encountered with MDT alone, although it did accelerate bacteriological clearance, histopathological upgrading, conversion to lepromin positivity, and clinical improvement. (AU)^ien. Descriptors: Hanseníase/quimioter Hanseníase/terap Mycobacterium leprae/genet Mycobacterium leprae/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n3/v67n3a04.pdf / en Location: BR191.1

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Id: 19644 Author: Ebenezer, Gigi J; Arumugam, Shantha; Job, Charles K. Title: Infection by M. leprae is governed by the temperature at the entry point: a preliminary note. Source: Int. J. Lepr;67(2):162-164, Jun., 1999. ilus, tab. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n2/v67n2cor01.pdf / en Location: BR191.1

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Id: 19639 Author: Sachdeva, Geetanjali; Kaur, Gurvinder; Bhutani, L. K; Bamezai, R. N. K. Title: Lymphoproliferative responses of leprosy patients and healthy controls to nitrocellulose-bound M. leprae antigens. Source: Int. J. Lepr;67(2):133-142, Jun., 1999. tab, graf. Abstract: The lymphoproliferative responses of 51 leprosy patients and 11 healthy contacts were analyzed using the nitrocellulose-bound specific antigen fractions from the cell-free extract of Mycobacterium leprae. The main proliferation-inducing fraction for peripheral blood mononuclear cells of the healthy contacts was found to be the Fraction II, bearing antigens in the range of 66-45 kDa. However, this fraction failed to induce lymphoproliferation in the leprosy patients, unlike healthy contacts (p < 0.032). The number of responders as well as the strength of the responses to 66-45 kDa proteins were found to be low in the leprosy patients compared to the healthy contacts. Further, preliminary analysis with the subfractions of Fraction II produced a similar pattern, suggesting that the immune response to the antigens in the range of 66-45 kDa M. leprae proteins remains suppressed in subjects with clinical signs and symptoms of the disease. (AU)^ien. Descriptors: Hanseníase/genet Hanseníase/imunol Colódio/anal Colódio/uso diag Mycobacterium leprae/genet Mycobacterium leprae/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n2/v67n2a03.pdf / en Location: BR191.1

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Id: 19627 Author: Sakai, Tetsuo; Matsuo, Eiichi; Wakizaka, Akira. Title: Complete DNA sequence analysis for 16s ribosomal RNA gene of the leproma-derived, cultivable and nerve-invading mycobacterium HI-75. Source: Int. J. Lepr;67(1):52-59, Mar., 1999. tab. Abstract: The complete 1493 nucleotide sequence of the 16SrRNA gene of the leproma-derived and cultivable mycobacterium HI-75 strain was analyzed to elucidate the taxonomic characteristics by direct sequencing of the polymerase chain reaction (PCR) products. The results revealed that the sequence of mycobacterium HI-75 was mostly similar to that of Mycobacterium scrofulaceum with 5 bases differences in the sequenced 1493 bases (0.35%) of the 16SrRNA gene. M. leprae differed from the strain with 47 bases (3.3%). Sasaki and Hamit reported the nerve-invasive activity of the inoculated mycobacterium HI-75 in nude mice or the 131I-treated immunocompromised Swiss mice. The results indicate that mycobacterium HI-75 could be a mutant of M. scrofulaceum possessing the ability to invade the peripheral nerve in addition to developing leproma-like lesions. (AU)^ien. Descriptors: DNA/genet RNA Ribossômico/genet RNA Ribossômico/imunol Mycobacterium leprae/genet Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/1999/pdf/v67n1/v67n1a08.pdf / en Location: BR191.1

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Id: 19549 Author: Sharma, Pankaj; Kar, Hemant K; Kaur, Harvinder; Misra, Radhey S; Mukherjee, Ashok; Mukherjee, Rama; Rani, Rajni. Title: Induction of lepromin positivity and immunoprophylaxis in household contacts of multibacillary leprosy patients: a pilot study with a candidate vaccine, Mycobacterium w. Source: Int. J. Lepr;68(2):136-142, Jun., 2000. tab. Abstract: We screened 487 household contacts of multibacillary (MB) patients for evidence of disease and their lepromin status. From the 444 results available, 302 (68.02%) were lepromin positive and 142 (31.98%) were lepromin negative on initial testing. The initial lepromin status as assessed in the group of 54 contacts having disease at the outset showed 24 out of 46 (52.2%) to be lepromin positive and 22 of 46 (47.8%) to be lepromin negative. In the same group, among 24 lepromin positives, 22 (91.7%) had paucibacillary (PB) and 2 (8.3%) had multibacillary (MB) disease; among the lepromin negatives, 12 (54.5%) had PB and 10 (45.5%) had MB disease. Out of 72 initially lepromin-negative contacts administered Mycobacterium w vaccine and followed up, the cumulative percentages show that 53 (73.6%) converted to positivity after a single dose, 10 (87.5%) after a second dose and 67 (93.1%) after the third dose. The incidence of new cases with leprosy was 8 out of 231 (3.46%) among lepromin-positive contacts and 5 out of 93 (5.38%) among lepromin-negative contacts administered Mycobacterium w vaccine. Among 231 lepromin-positive contacts, the new cases occurred in those with a 1+ and 2+ lepromin response only, and no case occurred among 51 contacts with a 3+ lepromin response. The incidence among lepromin-positive contacts in this study (3.46%) was similar to the observations in two other studies: 3.2% by Dharmendra, et al. and 6.9% by Chaudhary, et al. However, the incidence among lepromin-negative contacts administered Mycobacterium w vaccine was significantly lower than that observed among lepromin-negative contacts not administered any vaccination in the other two studies (14.1% by Dharmendra, et al. and 29.0% by Chaudhary, et al.). To conclude, although a study of small sample size, the preliminary evaluation indicates that administration of Mycobacterium w vaccine seems to have the potential to reduce the incidence of leprosy among household contacts of leprosy patients. More explicit results about the vaccine will be available from the ongoing field trials in Kanpur Dehat in the near future. (AU)^ien. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Hanseníase Dimorfa/imunol Hanseníase Virchowiana/imunol Hanseníase Tuberculóide/imunol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2000/pdf/v68n2/v68n2a03.pdf / en Location: BR191.1

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Id: 19547 Author: Matsuoka, Masanori; Maeda, Shinji; Kai, Masanori; Nakata, Noboru; Chae, Gue-Tae; Gillis, Thomas P; Kobayashi, Kazuo; Izumi, Shinzo; Kashiwabara, Yoshiko. Title: Mycobacterium leprae typing by genomic diversity and global distribution of genotypes. Source: Int. J. Lepr;68(2):121-128, Jun., 2000. tab, graf, mapa. Abstract: The genetic diversity and related global distribution of 51 Mycobacterium leprae isolates were studied. Isolates were obtained from leprosy patients from 12 geographically distinct regions of the world and two were obtained from nonhuman sources. Polymerase chain reaction (PCR) followed by DNA sequencing was performed targeting the rpoT gene of M. leprae. Isolates were classified into two groups based on the number of tandem repeats composed of 6 base pairs in the rpoT gene. Isolates from Japan (except Okinawa) and Korea belonged to one group, while those from Southeast Asian countries, Brazil, Haiti and Okinawa in Japan belonged to a second genotype. M. leprae obtained from two nonhuman sources (an armadillo and a mangabey monkey) revealed the latter genotype. These results demonstrate the genetic diversity of M. leprae and the related genotype-specific distribution in the world. (AU)^ien. Descriptors: Mycobacterium leprae/genet Genoma/genet Genótipo Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2000/pdf/v68n2/v68n2a01.pdf / en Location: BR191.1

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Id: 19421 Author: Parkash, Om. Title: A study on the reproducibility of two serological assays for detection of Mycobacterium leprae infection. Source: Int. J. Lepr;69(1):46-48, Mar., 2001. tab. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/imunol Mycobacterium leprae/fisiol Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1cor06.pdf / en Location: BR191.1

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Id: 19419 Author: Salamatina, Olga S; Dyachina, Margarita N; Juscenko, Anatoly A; Parshin, Michail P; Chernoyusova, Larisa N. Title: Comparative characteristics of antigenic profile of M. leprae and M. lufu. Source: Int. J. Lepr;69(1):39-43, Mar., 2001. ilus, tab. Descriptors: Mycobacterium leprae/genet Mycobacterium leprae/ultraest Electronic Medium: http://hansen.bvs.ilsl.br/textoc/revistas/intjlepr/2001/pdf/v69n1/v69n1cor04.pdf / en Location: BR191.1

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